Cell Mechanosensing-3: Yui set up the Multipurpose Small Payload Rack (MSPR) Experiment Laptop Terminal (ELT) in preparation for Cell Mechanosensing-3 operations that will be performed after the arrival of investigation samples on the SpaceX-8 in January, 2016. He exchanged the laptop hard drive and installed cables between the MSPR Small Experiment Area and the laptop. Identification of gravity sensors in skeletal muscle cells during Japan Aerospace Exploration Agency’s (JAXA’s) Cell Mechanosensing investigation will support development of countermeasures against muscle atrophy, a significant health issue for humans in space. Scientists believe that the lack of mechanical stress from gravity causes tension fluctuations in the plasma membrane of skeletal muscle cells, which changes the expression of key proteins and genes and allows muscles to atrophy. Muscle cells from rats and kidney cells from African clawed frogs are tagged with fluorescent gene markers and attached to an extracellular matrix to study their performance under different tensions that simulate their use on earth. Observation and Analysis of Smectic Islands in Space (OASIS) Glycerol/Water Fill: Due to a malfunction with the Microgravity Science Glovebox (MSG) slide mechanism that allows the MSG work volume to be extended for crew access, Kononenko was unable to inject glycerol and water into the current OASIS sample under study. While an investigation had begun to resolve the MSG problem, the OASIS ground experts decided to continue the investigation with the untreated OASIS sample. OASIS studies the unique behavior of liquid crystals in microgravity, including their overall motion and the merging of crystal layers known as smectic islands. Liquid crystals are used for display screens in televisions and clocks, and they also occur in soaps and cell membranes. The experiment allows detailed studies of the behavior of these structures, and how microgravity affects their unique ability to act like both a liquid and a solid crystal. Sleep Log: Kornienko recorded a Sleep Log entry today. The Sleep ISS-12 experiment monitors ambient light exposure and crew member activity and collects subjective evaluations of sleep and alertness. The investigation examines the effects of space flight and ambient light exposure on sleep during a year-long mission on the ISS. Extravehicular Activity (EVA) Tool Stow: Kelly and Lindgren stowed tools which were used earlier this month as part of the P6 Return To Original Configuration (RTOC) EVA #33. Soyuz 43 Imagery: Yui configured a D4 camera in order to obtain high resolution imagery of the Soyuz 43 Descent Module, currently docked to Mini-Research Module (MRM)-1. The images will be downlinked to the ground and used for Micrometeoroid/Orbital Debris (MMOD) risk mitigation. Lab Window Cleaning: Lindgren cleaned the inboard surface of LAB Primary Pressure Pane. The cleaning was performed in preparation for the METEOR Payload which will be installed in the Window Observational Research Facility (WORF) targeted to arrive early 2016 onboard SpaceX-9. Mobile Servicing System (MSS) Operations: Overnight, Robotics Ground Controllers powered up the MSS and performed a survey of the Functional Cargo Block (FGB) Starboard Solar Array Wing (SAW). FGB SAW surveys will continue tomorrow. Today’s Planned Activities All activities were completed unless otherwise noted. Biochemical Urine Test SPLANH. REFLOTRON-4 Analyzer Setup, Biochemistry Study, Start EGEG Recording SPLANH. Photography of the Experiment Ops Biochemical Urine Test URISYS Hardware Stowage Leak isolation in Orlan-MK suit 4 and suit 6 backup bladder MSPR – Multi-Purpose Small Payload Rack (MSPR) Hard Drive Changeout OCT Hardware Setup US EVA Tool Stow MSPR ELT MSPR Hardware Activation WRS – Recycle Tank Fill Filling (separation) of EDV (KOV) for Elektron or EDV-SV. Inspect and separate EDV Optical Coherence Tomography (OCT) СОЖ Maintenance SPLANH. Blood Biochemistry Analysis OCT Hardware Stowage Spacesuit and БСС (Orlan Interface Unit) leak checks and Orlan 5 valve test Start BSA Battery Charge Orlan Suit 5 Backup Bladder Leak Check Verification of ИП-1 Flow Sensor Position HABIT – Video during the Experiment [Deferred] OASIS – Filling container with water and glycerol US EVA Tool Stow WRS – Recycle Tank Fill [Deferred] Soyuz 717 Photos from DC1 and Cupola Lab Window Cleaning MELF2 – Ice Brick Insertion into MELFI Test activation of Vozdukh Atmosphere Purification System Emergency Vacuum Valves [АВК СОА] Recovery of Orlan 4 ventilation system Environmental Health System (EHS) – Intravehicular Tissue Equivalent Proportional Counter (IV-TEPC) Relocate IMS Delta File Prep CIR – Hardware Setup INTERACTION-2. Experiment Ops MDCA – Preparation of MWA Surface Area Repairs of SM Interior Panel 310 by Installing Overlay Panels – Day 2 Preparation for diagnostics of power supply system (СЭС) and onboard complex control system (СУБК) units in FGB, review of crew procedures and radiograms HAM radio session from Columbus PAO Event with the 1 Year Mission crew US EVA Tool Stow HRF1- Data Export MSPR – Payload Closeout Ops Fundoscope Exam Setup (Ophthalmoscope) TWIN – Hardware Setup Preparing for БИТС2-12 ДКЦ2Б15 Device Replacement (locating equipment, r/g review) Fundoscope Examination Start BSA Battery Charge Equipment stowage after Fundoscope Eye Imaging (Ophthalmoscope) Preparation of Reports for Roscosmos Web Site and Social ECON-M. Observation and Photography URAGAN Observations and Photography Completed Task List Items Robot Startup WHC KTO Replace JLP Hardware Consolidate Ground Activities All activities were completed unless otherwise noted. Nominal Commanding Three-Day Look Ahead: Wednesday, 11/18: Ocular Health, Port Solar Array Mast Survey, FGB Diagnostics Thursday, 11/19: C2V2 Aft Cable Routing, Haptics 1 Friday, 11/20: PEPS Audit, SSRMS LEE-B Inspection, MPEP Removal from JEMAL Slide Table QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) On [СКВ] 2 – SM Air Conditioner System (“SKV2”) Off Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Shutdown Major Constituent Analyzer (MCA) Node 3 Manual Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Full Up Trace Contaminant Control System (TCCS) Node 3 Off
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