SPHEROIDS Temperature and Power Check: The ESA SPHEROIDS experiment completed its two day experiment run. Yesterday, Peake retrieved the SPHEROIDS hardware from SpaceX-8 (SpX-8) and installed the containers into the Kubik. Today he will completed a temperature and power check. The experiment investigates the effects of microgravity on endothelial cell function with respect to blood vessel formation, cellular proliferation, and programmed cell death. Results could help in the development of potential countermeasures to prevent cardiovascular deconditioning in astronauts and improve knowledge of endothelial functions on Earth. Rodent Research-3 (RR-3) Transfer: Kopra and Peake set up the rodent habitats, moved the transporters from SpX-8 to the US Lab, and transferred the animals to the habitats. RR-3 is a Joint USOS Russian Experiment performed with both USOS and Russian crew members. RR-3 studies molecular and physical changes to the musculoskeletal system that happen in space. Results will expand scientists’ understanding of muscle atrophy and bone loss in space while testing an antibody that has been known to prevent muscle wasting in mice on Earth. Cell Mechanosensing 3 (CMS-3) Microscope Observation: Williams completed the first of three microscope observation settings. He retrieved the Measurement Experiment Culture Chamber from the Cell Biology Experiment Facility (CBEF) Incubator Unit (IU) and installed in microscope stage for observation by the ground. CMS-3 is a JAXA investigation that identifies gravity sensors in skeletal muscle cells to develop countermeasures to muscle atrophy. Scientists believe that the lack of mechanical stress from gravity causes tension fluctuations in the plasma membrane of skeletal muscle cells which changes the expression of key proteins and genes and allows muscles to atrophy. NanoRacks Module-51: Williams watched an operations overview video and retrieved NanoRack Module 51 from the Minus Eight-degree Freezer for ISS (MELFI) Rack before initiating the NanoRacks Mission Discovery-2 sub-experiments housed within NanoRacks Module-51. The four different investigations within the module study: antibiotic use to inhibit Staphylococcus aureus bacteria; the rate at which yeast decomposes organic matter; whether steroid-enhanced plants could grow better in space than on Earth; and whether an enzyme derived from fireflies can cause bioluminescence in microgravity. Multi-Omics Operations: Williams performed the Japan Aerospace Exploration Agency (JAXA) Multi-Omics investigation by collecting human waste samples and inserting them into a Box Module in the MELFI (Minus Eight-degree Freezer for ISS). The investigation evaluates the impacts of space environment and prebiotics on astronauts’ immune function by combining the data obtained from the measurements of changes in the gut microbiological composition, metabolites profiles, and the immune system. Ultrasound 2 Operations: Williams performed an Ultrasound software load by updating Ultrasound 2 system build software using the Ultrasound 2 Boot Drive. The software update supports the Fluid Shifts activities next week. Orbital-ATK 6 (OA-6) Cargo Operations Status: The crew completed cargo operations off the task list today, for a total of 18:35 of OA-6 cargo ops. SpaceX-8 Cargo Operations Status: The crew completed cargo operations off the task list today, for a total of 3:50 total unpack operations since ingress yesterday. Today’s Planned Activities All activities were completed unless otherwise noted. EarthKAM. Battery Swap WRS Water Sample Analysis NanoRacks Module-51 – Retrieval from MELFI Handheld HDPCG unit and nine PCG Cards retrieved from Polar, stowed in +4 Double Coldbag, remaining samples are transferred to MELFI SPHEROIDS – KUBIK Temperature Check Setup and Activation of Crew Onboard Support Kit (КСПЭ) Equipment for MPEG2 TV coverage from SM Microgravity Science Glovebox (MSG) Activation with LSAH Audio Session with “This is Radio Moscow” radio station r/g 1934 Multi Omics Fecal – Operations with Samples Crew Prep for PAO Multi Omics (MO) – Sample Insertion into MELFI Multi Omics (MO) – Equipment Stowage after Sample Collection Test Session ISS-Kremlin-Vostochny Cosmodrome (S + Ku-band) / Video & Audio Rodent Research Experiment Camcorder Installation in Node 2 HRF Rack 1 -Ultrasound 2 Hardware Setup Rodent Research Transfer 1 Crew Prep for PAO Telebridge ISS-Kremlin-Vostochny Kosmodrome Deactivation of Camcorder, Video Control Monitor [ВКУ], and Closing CP SSC Applications NanoRacks Module-51 Overview NANO – Start Experiment PAO Hardware Setup ABOUT GAGARIN FROM SPACE. Hardware deactivation / r/g 1914 Crew Prep for PAO / r/g 1935, 1930 Press Conference with TASS Information Agency r/g 1935 TV Conference with Life News TV Channel r/g 1930 ISS HAM – HAM radio session with students EarthKAM. Battery Swap USND2 – SW Update ABOUT GAGARIN FROM SPACE. Hardware activation / r/g 1914 TOCA Data Recording Crew Prep for PAO / r/g 1933 TV Conference with the Participants of the Pan-Russian Let’sGo! session in Artek International Children Center r/g 1933 Rodent Research: Transfer 2 PCG4 – Sample Preparation, Activation, and Setup in MELFI Stowage of Double Coldbags and Icebricks USND2 – Closeout Ops СОЖ Maintenance CMS3 – MICROSCOPE Sample Observation Water Recovery Management (WRM) – Water Consumption Balance, place holder USND2 – Hardware Deactivation MSG – Hardware Deactivation EarthKAM. Battery Swap Completed Task List Items None Ground Activities All activities were completed unless otherwise noted. RR transfer SPDM unstow from MBS PDGF2 for FGB Port SAW survey Nominal ground commanding Three-Day Look Ahead: Wednesday, 04/13: Ocular Health, NanoRacks Module 9 ops, N3 aft CBCS install/checkout, EPO-Biorock, SPRINT Ultrasound Thursday, 04/14: Ocular Health, Lab KU power supply install, universal battery charger install/checkout, bone densitometer calibration, CMS3 ops, SPRINT Ultrasound Friday, 04/15: Ocular Health, CMS3 ops, SODF deploy, Smartcycler install, WHC urine receptacle R&R QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Override Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Off Trace Contaminant Control System (TCCS) Node 3 Full Up
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