Magnetic 3 Dimensional (Mag 3D) Cell Culturing: The crew prepared hardware in the Microgravity Science Glovebox (MSG) for Mag 3D operations. BioCell Habitat, Mag 3D samples and Cultures Inoculation Kits were removed from the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) and used to inoculate Multiwell BioCells using Inoculum syringes. Cell cultures in space spontaneously grow in three dimensions, which results in characteristics more representative of how cells grow and function in living organisms. But in microgravity, routine manipulation of cell cultures is challenging. This investigation uses magnetized cells and tools to make it easier to handle cells and cultures and to improve the reproducibility of experiments. This approach also makes it possible to generate two-dimensional cultures as controls, and to determine whether biological events in these monolayer cultures result from gravity or substrate attachment. Japanese Experiment Module Airlock (JEMAL) Operations: The crew pressurized the JEMAL this morning and performed a leak check. This is in preparation for installation of the Handhold Experiment Platform (HXP) adapter on the JEMAL Slide Table later this week that will support the Exposed Experiment Handhold Attachment Mechanism (ExHAM) investigation operations planned for next week. Capillary Structures for Exploration Life Support (Capillary Structures): The crew set up hardware for the Capillary Structures investigation and completed two sorbent demonstrations. They first demonstrated flow through two microgravity air-liquid contactors in series, then demonstrated flow through two parallel microgravity air-liquid contactor wedges with a viscous fluid. Current life-support systems on the ISS require special equipment to separate liquids and gases including rotating or moving devices that could cause contamination if they break or fail. Capillary Structures studies a new method using structures of specific shapes to manage fluid and gas mixtures. It also studies water recycling and carbon dioxide removal, benefitting future efforts to design lightweight, more reliable life support systems for future space missions. On-Board Training (OBT) 50 Soyuz (50S) Emergency Egress Drill: All three crew members participated in this OBT to practice procedures for departing the Station in the event of an emergency. This drill is scheduled 12 to 14 weeks aboard the ISS and every 2.5 months thereafter. Today’s Planned Activities All activities were completed unless otherwise noted. Test Video Recording for Russia Today TV Channel VIZIR. Modification of SM panels 114, 116 for СКПФ-УМ P/L URAGAN. Observation and Photography ECON-M. Observation and Photography Combustion Integrated Rack Alignment Guide Removal ISS Emergency descent drill. JEM Airlock Pressurization Magnetic 3D Cultures Thawing and Inoculation Preparation WHC Full Fill Magnetic 3D Cultures MELFI Sample Retrieve JEM Airlock Leak Check TOCA Water Recovery System (WRS) Sample Analysis Mating telemetry data connector БСК-1В. БСК-1В Power Switching Unit R&R in Regul-OS Onboard Radio Complex (БРТК – Closeout Ops.) Rodent Research Water Box and Light Check Auxiliary Laptop Computer System Virus Definition File Update Tropical Cyclone Untended Operations СОЖ maintenance Public Affairs Office (PAO) High Definition (HD) Config Columbus Setup HRF1 PC 1 Boot Drive Update Magnetic 3D Cultures Biocells Inoculation Magnetic 3D Cultures Session Cleanup TOCA Sample Data Record RGN Wastewater Storage Tank Assembly (WSTA) Fill WORF Monthly Laptop Connect Glacier Desiccant Swap Tropical Cyclone Hardware Closeout Extravehicular Metal Oxide (METOX) Regeneration Initiation and Verification Completed Task List Items Capillary Structures Sorbent Contactor Demonstration Ground Activities All activities were completed unless otherwise noted. JEMAL pressurization/leak check Three-Day Look Ahead: Tuesday, 07/11: N3 CCAA water separator R&R, Magnetic 3D Cultures microscope ops, Max CEVIS portable PFS setup Wednesday, 07/12: Max CEVIS session, TPF experiment setup, ELF sample holder exchange, RR Habitat restock Thursday, 07/13: WORF PEHG/Shutter actuator system install, JEMRMS cable reconfig for BDS checkout, RR access unit cleaning QUICK ISS Status – Environmental Control Group: Component Status Elektron Off Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Operate Carbon Dioxide Removal Assembly (CDRA) Node 3 Standby Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Standby Urine Processing Assembly (UPA) Process Trace Contaminant Control System (TCCS) Lab Full up Trace Contaminant Control System (TCCS) Node 3 Off
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