CREAM (Cosmic Ray Energetics and Mass): Last night the CREAM external payload was removed from the SpX-12 trunk by the Space Station Remote Manipulator System (SSRMS) and handed off to the Japanese Experiment Module Remote Manipulator System (JEMRMS), which then installed it on Exposed Facility Unit-2 (EFU-2) on the JEM Exposed Facility (JEF). Ground controllers then commanded activation of the CREAM payload. CREAM’s instruments measure the charges of cosmic rays over a broad energy range for a planned three years of operation on the ISS. The origins of cosmic rays and the mechanisms that accelerate them to high speeds are among the oldest questions in modern astrophysics. Results from CREAM are expected to bring the science community closer to answering those questions and build a stronger understanding of the fundamental structure of the universe. Cardio Ox Ultrasound: A 50S crewmember completed their Return minus 15 Day (R-15) ultrasound, blood pressure and Electrocardiogram (ECG) measurements for the Cardio Ox investigation. Another crewmember provided scanning assistance after the subject crewmember donned the ECG electrodes and marked the carotid and brachial arteries for scanning. By collecting ultrasound and ECG data, along with blood and urine samples, scientists are trying to determine whether biological markers of oxidative and inflammatory stress are elevated during and after space flight and whether this results in an increased, long-term risk of atherosclerosis in astronauts. ADvanced Space Experiment Processor (ADSEP): The crew removed Cell Culturing (CellCult) cassettes from ADSEP, inserted cells into each cassette and reinserted them into ADSEP. ADSEP is a thermally controlled facility that accommodates up to three cassette-based experiments that can be independently operated. Its companion hardware consists of a collection of several experiment cassettes that accommodate experiments in cell technology, model organisms, multiphase fluids, solution chemistry, separation science, microencapsulation, and crystal growth. For CellCult investigations, each cassette contains a rotating filtered bioreactor, a reservoir for fresh media, two peristaltic pumps, a waste reservoir, and up to 6 sample-collection or reagent containers connected by a manifold to the reactor. Cultures can be operated in continuous perfusion, batch fed, static, or sampling mode. The removal of samples and the addition of additives to the reactor volume can be programmed or teleoperated. Multi-Omics-Mouse: The crew performed routine maintenance for the Multi-Omics-Mouse Cage Units, exchanging waste collection equipment and odor filters. Several studies have reported space flight effects on the human immune system, but the relationship between microbiota and immune dysfunction during flight remains unclear. In the Multi-Omics-Mouse investigation, food with and without fructooligosaccharides (FOS) will be used as prebiotics, to determine if they improve the gut environment and immune function. After the flight, researchers will analyze the gut environment (microbiota and metabolites) and immune system of the mice by multi-omics analysis. Bigelow Expandable Activity Module (BEAM): The crew ingressed BEAM and collecteded air and surface microbial samples. BEAM is an experimental expandable module attached to the ISS. Expandable modules weigh less and take up less room on a rocket than a traditional module, while providing additional space on-orbit for living and working. Crews traveling to the moon, Mars, asteroids, or other destinations may be able to use them as habitable structures. Treadmill 2 (T2): This afternoon the crew reported a clicking noise emanating from T2 that had not been heard previously. The crew took video and audio which were downlinked for ground team evaluation. T2 is currently no-go for exercise. Today’s Planned Activities All activities were completed unless otherwise noted. Advanced Space Experiment Processor Cell Collection On-Board Training Advanced Space Experiment Processor Cell Collection ADSEP MELFI Sample Insertion #1 ADSEP MELFI Removal #2 AQUAPAD Training KUBIK Functional check BEAM Cleanup and Egress BEAM Ingress Bigelow Expandable Activity Module (BEAM) Node 3 Aft Stow/Unstow Cardio Ox CDL Holter Arterial BP Hardware Doff – Subject Cardio Ox CDL Holter Arterial BP Measurement – Subject Cardio Ox Ultrasound Preparation Cardio Ox Ultrasound Scan – Operator Cardio Ox Ultrasound Scan – Subject Combustion Integrated Rack Alignment Guide Install ARED Platform Fold ARED Platform Unfold Back to Nominal Position Crew Departure Preparations for Return to Earth Environmental Health System (EHS) – Microbial Air Sampler (MAS) Kit Sample Collection Environmental Health System (EHS) – Surface Sample Kit (SSK) Collection/Incubation Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record Extravehicular Mobility Unit (EMU) Post Scrub Cooling Loop Water (H2O) Sample Stow Syringes used in H2O Conductivity Test Extravehicular Mobility Unit (EMU) Conductivity Test EVA Extravehicular Mobility Unit (EMU) Cooling Loop Scrub Deconfiguration Extravehicular Mobility Unit (EMU) Cooling Loop Maintenance Scrub Initiation Extravehicular Mobility Unit (EMU) Cooling Loop Maintenance Iodination Handover Feedback Questions Health Maintenance System (HMS) ISS Medical Accessories Kit (IMAK) Unpack from SPX-12 Health Maintenance System (HMS) Tonometry Test Setup/Stow Health Maintenance System (HMS) Tonometry Test Crew Medical Officer (CMO) Health Maintenance System (HMS) Tonometry Test Subject Health Maintenance System (HMS) Vision Questionnaire Health Maintenance System (HMS) Vision Test IFUNGUS sample MELFI Insertion #1 Auxiliary Computer System [ВКС] Hardware Audit Lighting Effects Sleep Log Entry – Subject Multi-Omics-Mouse Cage Cleaning Familiarization Conference Multi-Omics-Mouse Cage Cleaning and Maintenance for Food 1G first part 1 Multi-Omics-Mouse Cage Cleaning and Maintenance for Food 1G first part 2 Multi-Omics-Mouse Item Gathering Photo/TV N3/BEAM Camcorder Setup Probiotics Fecal Sample MELFI Insertion Probiotics Fecal Sample Operations Probiotics Sample Photo Reminder Rodent Research Thermal Consideration Treadmill 2 (T2) Photo/TV Reminder Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill Наклейка русской клавиатуры на лэптоп RSК2 Pre-pack cargo items for return or disposal on Soyuz 735 Surface Tension Containment MWA Assist Surface Tension Containment MWA Operations Surface Tension Containment MWA Preperation Ultrasound 2 HRF Rack 1 Power On Water Recovery System Waste Water Tank Drain Init Water Recovery System Waste Water Tank Drain Termination XF305 Camcorder Setup Test video recording for RT channel Stop discharging the 1st Orlan Battery Pack 825М3 and and start discharging the 2nd Battery Pack EVA tool stow, update IMS RSK1 Laptop Replacement DUBRAVA. 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